HEK Expression Cell Culture Medium
High-Performance Chemically-Defined Media for HEK293 Cell Culture
HEK Expression Cell Culture Medium
CliniSciences offers high-performance media for HEK293 cells. The media are perfectly suitable for research and further manufacturing.
Advantages at a Glance
✓ Supports growth of high-density HEK293 cultures
✓ Enables sustained, high-level expression of recombinant protein
✓ Promotes transfection efficiency and production
High Yield Human Expression System
The human embryonic kidney cell line HEK293 has biosynthetic potential for human-like production and is nowadays used for manufacturing of several therapeutic proteins and viral vectors. While initially used for adenoviral vector production, HEK293 also became one of the preferred cell lines for transient or stable protein expression. The need for proper protein folding and glycosylation of therapeutic proteins has promoted the production in HEK293.
Products
HEK|ONE S
Medium for Stable Expression in Suspension Culture
The product HEK|ONE S is the right cultivation and production medium for stable HEK293 cell lines. It supports stable growth of suspension cells at high viability in seed train culture. HEK|ONE S promotes the production of recombinant proteins and antibodies in suspension culture.
Cat-No.: HEKS-1000ML | Volume: 1 L
Storage: +2°C to +8°C | Sterile-filtered
HEK|ONE T
Medium for Transient Expression and Efficient Transfection
The medium HEK|ONE T was designed for high-density suspension cultures and efficient transfection. Furthermore, it is highly suitable for transient protein expression and viral vector production in HEK293 and other human cell lines. Use the same medium for seeding, transfection, and production.
Cat-No.: HEKT-1000ML | Volume: 1 L
Storage: +2°C to +8°C | Sterile-filtered
HEK|ONE Feed
Feeding Supplement for Extended Culture & High Yields
Chemically-defined, concentrated nutrient formulation
Enhances protein and antibody yield in both stable and transient systems
Maintains cell viability and production phase
Compatible with HEK|ONE S and HEK|ONE T
Cat-No.: HEKF-1000ML | Volume: 1 L
Customizable feeding strategy from Day 2 depending on cell metabolism.
Data-Driven Performance
Transfection Efficiency (GFP Assay)
| Medium | Transfection Efficiency |
|---|---|
| HEK|ONE T | ~80–90% |
| Commercial Media 1 | ~60–70% |
| Commercial Media 2 | ~40–50% |
✓ Proven superior performance in Expi293 and HEK F cells
Viable Cell Density & Viability (Feeding Regime)
Stable performance for up to 14 days
10–12% daily feed addition recommended for high-yield outcomes
Outperforms two commercial alternatives in long-term culture trials
Custom Manufacturing & Scalability
Thanks to advanced production capabilities and ISO-certified processes, all HEK|ONE products are available in:
- Ready-to-use liquid format
- Powder media (100 L to 40,000 L batch sizes)
- Custom packaging in 2D/3D bioprocess bags (up to 1000 L) for GMP workflows
Perfect for labs, CMOs, and manufacturers seeking a scalable, consistent expression system.
Ordering & Samples
| Product | Format | Volume | Cat-No. |
|---|---|---|---|
| HEK|ONE S | Liquid | 1 L | HEKS-1000ML |
| HEK|ONE T | Liquid | 1 L | HEKT-1000ML |
| HEK|ONE Feed | Liquid | 1 L | HEKF-1000ML |
✓ Contact us to request a free sample, bulk pricing, or technical support.
Request a SampleWhy Choose CliniSciences?
As an experienced partner in bioproduction and life science solutions, CliniSciences brings you cutting-edge technologies from trusted suppliers like Capricorn Scientific. Our team provides:
✓ Tailored technical support
✓ Fast access to samples and documentation
✓ Regulatory guidance for GMP-readiness
✓ Seamless integration of cell culture workflows
At CliniSciences, we recognize the pressures faced by researchers and bioproduction teams in vaccine development and manufacturing for speed, scalability, and compliance. Our partnership with Capricorn Scientific enables us to deliver innovative solutions like the HEK|ONE Media System.