Protein analytics

Precise characterization of recombinant proteins is fundamental to their successful application in biopharmaceutical development, diagnostics, and academic research. At CliniSciences, we offer a comprehensive suite of analytical services to evaluate proteins produced through diverse expression and purification methods. Our advanced assay platforms provide detailed insights into protein activity, stability, and structural integrity, addressing critical challenges such as post-translational modification (PTM) heterogeneity, aggregation risks, and regulatory compliance. By leveraging cutting-edge technologies, including high-resolution mass spectrometry and real-time binding assays, we ensure that your proteins meet stringent quality standards for downstream applications.

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Our Analytical Services

We offer a range of analytical methods utilizing multiple assay platforms that be used to characterize a broad range of proteins expressed and purified by various methods.

Our team will work closely with you from concept through delivery. Together, we'll develop a set of project deliverables tailored to your specifications of scale, purity, activity and other analytical characteristics on a timeline that meets your needs.

You will find below the various services that we offer. Please contact us at tech@clinisciences.com for more information or for a quote request.

Protein Activity

Assessing protein function is essential for validating biological activity. Our activity assays include:

Binding affinity (FortéBio Octet® RED96 System)
Enzyme activity
Cell-based assays
In vivo testing

Aggregation Status

Monitoring aggregation ensures protein quality and efficacy. Our methods include:

SDS-PAGE
SEC-HPLC
Optical density

Protein Stability

Stability analysis is critical for formulation and storage. Our stability assays include:

Tm&Tagg
ΔG
Isothermal stability
Thermal recovery
Sizing&polydispersity
Sizing with thermal ramp
B22&kD
Viscosity

Protein Integrity and Structure

Structural characterization verifies protein quality and PTMs. Our techniques include:

SEC-HPLC
SDS-PAGE
N-glycan analysisWestern Blot

Protein mass (Agilent HPLC-CHIP/QTOF)

Accurate mass: proteins, protein-drug conjugates
Peptide and Glycan mapping
Determination of post-translations modifications either in known or unknown samples.

Standard COAs

Each project includes comprehensive Certificates of Analysis (COAs), documenting protein purity, activity, and structural characteristics to ensure compliance with research and regulatory standards.