At CliniSciences, we are proud to offer a complete line of hybridoma cell culture media and supplements, developed to meet the complex demands of diagnostic and biopharmaceutical manufacturers. With our high-performance solutions such as HybridomaPlus™ and the Hybridoma Supplement, your team can achieve high-yield monoclonal antibody (mAb) production with improved process control, consistency, and scalability.

Hybridoma Technology: Still a Cornerstone in mAb Development

Over 45 years since its introduction, hybridoma technology remains a cornerstone in monoclonal antibody research and manufacturing. By fusing a B-lymphocyte with a myeloma (tumor) cell, scientists produce a hybridoma—an immortal cell line capable of secreting highly specific antibodies.

While recombinant platforms are expanding, hybridoma-based methods continue to play a vital role in:

• Diagnostic antibody development
• Biosimilar and therapeutic screening
• Immunoassay manufacturing
• Research & development of novel antibody fragments

HybridomaPlus™: A Premium, Ready-to-Use Culture Medium

HybridomaPlus™ is a protein-free, serum-free, and animal origin-free medium formulated for both suspension and stationary hybridoma and myeloma cell cultures. Its chemically defined formulation avoids components that interfere with downstream processing, ensuring simplified purification and reproducibility.

Key Benefits of HybridomaPlus™:

• ✅ Free from animal and human components
• ✅ No serum, no proteins — ideal for antibody purification
• ✅ Supports fast initial cell growth and high antibody titers
• ✅ Adaptable for use in batch, fed-batch, and perfusion systems
• ✅ Compatible with traditional and recombinant myeloma lines

HybridomaPlus™ can also be supplemented for use as a serum-enriched medium if desired.

Product Specifications – HybridomaPlus™

Catalog No.: HY-A
Volume: 500 mL
Storage: +2°C to +8°C
Sterility: Sterile-filtered

Hybridoma Supplement: Replace Feeder Cells with Confidence

Our Hybridoma Supplement is a serum-free additive specifically designed to enhance cell viability and cloning efficiency after fusion. It replaces the need for feeder cells, eliminating common issues such as:

• Overgrowth of non-fused cells
• Contamination risks
• Nutrient competition
• Inconsistent growth factor levels

This supplement contains a proprietary blend of insulin, ethanolamine, hydrocortisone, retinoic acid, and other essential growth factors.

Hybridoma Supplement Highlights:

• ✅ Promotes hybridoma growth under serum-free conditions
• ✅ Enhances post-fusion survival and colony formation
• ✅ Low-protein formulation simplifies downstream purification

Product Specifications – Hybridoma Supplement

Catalog No.: HCS-F
Volume: 50 mL
Storage: ≤ -15°C
Sterility: Sterile-filtered

Why Partner with CliniSciences?

From early-stage antibody discovery to commercial-scale diagnostic kit manufacturing, CliniSciences offers expert support and advanced media formulations to optimize every step of your hybridoma workflow. Our team is ready to assist with:

• Process optimization
• Product selection and sampling
•  Custom packaging and formulation
• Regulatory documentation for QA/QC

Get in touch to request samples, technical datasheets, or to speak with one of our specialists about your antibody development strategy.